For Animal Feeds
What is PHYTEX 500
Phytase is an enzyme found in nature mainly in plants . It is formed
in the stage of sprouting to supply phosphorous during plant growth.
Phytase is also found in the small intestine mucous of many animals,
and in case of micro-organisms, it is found in Aspergillus sp. and
yeast. Its is obtained by a NON-GMO natural process from Aspergillus
Mode of action
Inositol Hexaphosphate , (IH), present in grains and beans, contain
one to three percent of (IH) , of which, 60% to 80% is phosphoric
acid. Wheat:0.6 to 1.4 % (w/w); Bran:2.6 to 5.4 % (w/w) ; Soy Flakes:1.5
to 1.8% (w/w) Prot. Concr: 2.0 to 3.0% (w/w) Phytase breaks down
the (IH) molecule releasing its phosphoric acid content making it
bioavailable to the animal, thus reducing the need for imorganic
PHYTEX 500 Savings in formulation,
economic benefits and units of activity
PHYTEX 500 when added to feed at the recommended levels, allows
for savings of 1.5% protein and 0.1 % phosphorous in formulation.
This represents a savings of at least 600% its cost of inlusion
per Mt of feed. One unit of activity of phytase in PHYTEX 500 is
the amount of enzyme required to liberate 1 micromole of inorganic
phosphorous per minute under the conditions of its assay method.
. One AGR U* (unit of activity) is the amount of enzyme required
to produce,together, an increase of 0.1% (w/w) of liberated phosphorous
and an increase of 1.5 % ( w/w) soluble protein in a gram of standard
formulated feed ration, at pH 5 , at 37ºC. PHYTEX contains 5000
AGR U* per gram.
Inositol Hexaphosphate (IH) , binds minerals, such as iron, calcium,
magnesium in feed, diminishing their bioavailability. It also forms
an undissolved complex with protein which prevents activity of digestive
enzymes, decreasing digestability of proteins and their functions,
such as emulsifying.Phosphoric acid in (IH) can not be used by single
stomach animals. Therefore, the need to add inororganic phosphate
in feeds as an additive. However, (IH) is secreted in feces, creating
environmental problems in large swine operations.
In the Netherlands, the use of poultry manure as a fertilizer is
regulated per regards to the amount of phosphate (P2O5) per acre.
Rate of application
100 grams per MT of feed. It can be pre-mixed or dispensed directly
into the mixer without pre-mixing. This inclusion rate provide 500,000
AGR U* units per Mt of feed.
Soluble in water:
Finisher 100 grms (Phytex 500) dissolved in 1000 Litres of water for 500 pigs
Grower 100 grms (Phytex 500) dissolved in 1000 Litres of water for 1000 pigs
Poultry 100grms(Phytex 500) dissolved in 1000 Litres of water for 10,000 Chickens.
Activity Vrs. Temperature: 100% Act.@ 60 ºC. ( Range 48ºC to 68ºC
) . Activity Vrs. pH : 100% Act. @ pH 6. (Range from 5.5 to 7).
However, PHYTEX 500 can be pelletized at maximum: 80 º C for 30
seconds. max with an aproxímate 5 % to 10% l enzyme loss. It is
100 % soluble in water. For maximum effect, add after pelleting.
PHYTEX 500 is packed for bulk shipments in 20 kg boxes lined with
special poly- plastic bag.It can also be packed in smaller polybags
as required by customer-
- The hydrolytic action of PHYTEX 500 on innositol hexaphosphate
increases the bio-availability of phosphorous, as well as other
nutrients in feeds. The presence of inositol hexaphosphate binds
minerals and forms an undissolved complex with proteins, diminishing
the bioavailability of nutrients in feeds. The breaking up of inositol
hexaphosphate by PHYTEX 500, not only releases phosphorous , but
a number of other nutrients , as expressed by the accompanying aminogram.
The values that appear in the amminogram are in terms of percentages
and only take into account the hydrolytic action of PHYTEX 500 .
The percentages are directly related to the PHYTEX 500 rate of inclusion
in the feed ( grams / Mt of feed).
In order to determine the % additional value of each nutrient made
available to the animal due to each enzyme's hydrolytic action ,
the following formula should be used:
Thus the nutrional value for each nutrient in % per Mt of feed equals:
% ) value in the amminogram X ( Dosage in grams ) PHYTEX 500
/ MT of Feed
Making this calculation for each nutrient in the amminogram , will
determine the total savings in formulation by PHYTEX 500 .
METHOD OF ASSAY TO DETERMINE AGRI*U
- An AGRI*U (Unit) is the amount of PHYTEX 500 required to produce,
together, an additional 1.5% of soluble protein and an additional
0.1% free phosphorous in feed rations.
- One gram of PHYTEX contains 5,000 units of AGRI*U
MODE OF ACTION
The hydrolytic action of PHYTEX 500 on inositol hexaphosphate (IH)
increases the bioavailabilty of phophorous , as well as proteins
in feed rations. Inositol hexaphosphate (IH) binds minerals and
undissolved complexes (that bind proteins), diminishing the bioavailability
of these proteins.in the animal gut. The breaking up of (IH) by
PHYTEX 500 releases free phosphorous and unbinds these protein,
making them more bioavailable to the animal. This action allows
for formulating rations with less phosphorous and less protein,
producing a savings in said formulation.
METHOD OF ASSAY
The object of the method of assay is to determine the increase of
free phosphorous and soluble protein in the ration in the ration.
1) Take one 2 kg of poultry
grower feed and mix in two separate jars , each, with a 1 liter
solution ( Ph 5) simulating the gastric juice composition.
2) In one jar, apply 1 (one)
gram of PHYTEX 500.
3) The other jar (control)
, stays blank.
4) Incubate both jars for
24 hours, at gut temperature while stirring mildly and continously
5) After 24 hours analyze
for free phosphorous by means of a spectrometer and for soluble
enzime functions of Amylase, Phytase and Protease
PHITEX 500 NEW PRESENTATION (.ppt)
Phytex 500 Formulation Guide
Phytex 500 Formulation
Matrix for Poultry
Phytex 500™ - University Trial “In Vivo”
National Autonomous University of Mexico - Augst 19th, 2004
500 University Trial in Pigs
Centro Nacional de Investigación en Fisiología Animal,
(CNIFA) INIFAP, Mexico.